Figure 4
From: IgE-activated mast cells enhance TLR4-mediated antigen-specific CD4+ T cell responses
TLR4 activation by LPS is required for enhanced antigen-specific CD4+ T cell response upon IgE/OVA-induced mast cell activation. (A,B) WT or TLR4-deficient mice were immunized with 2W1S peptide, IgE/OVA, LPS, or a combination of IgE/OVA and LPS. Cervical draining lymph nodes (A) and spleens (B) were analyzed 10 days post immunization for the numbers of 2W1S-specific CD4+ T cells. Results shown are cumulative data from 3 independent experiments n = 8–12 mice and shown as mean ± SEM. *P < 0.05 **P < 0.01, **P < 0.001, **** P < 0.0001. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparisons test.
