Figure 1
The evolution of body mass from weaning to sacrifice, across generations. (a) Body weight at weaning (g), expressed as Tukey’s whisker boxes (median, 25th to 75th percentiles ± 1.5 interquartile range). Mice of F0 Generation were weighted and then randomly assigned to an experimental group (n = 12 per group in each generation). Data was tested by one-way ANOVA with Tukey’s HSD for group comparison within the same generation. Two-way ANOVA with Šidak correction was used to compare each diet group in Generation F1 against its F2 counterparts. Significance was considered when p < 0.05. * vs. CTRL; # vs. HFD; § vs. Generation F1. Multiple significance signs represent different significance levels: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; filled circle Extreme values; + Group mean. Body weight curves for (b) F0 Generation, (c) F1 Generation and (d) F2 Generation were obtained by monitoring the mice weight weekly (12 mice per group in each generation). Results are expressed as the mean body weight (g), with whiskers representing the 95% Confidence Interval. The main and simple effects between groups were tested by Repeated Measures ANOVA corrected for family-wise comparisons using the Šidak method, and by Greenhouse–Geisser correction for sphericity. Graphs were obtained using the software GraphPad Prism 8 (San Diego, CA, USA). Significance was considered when p < 0.05. a—CTRL vs. HFD; b—CTRL vs. HFDt; c—HFD vs. HFDt. DR diet reversion.
