Figure 3
Sperm parameters across all the generations (n = 12 per group in each generation). (a) Sperm concentration; (b) sperm motility; (c) sperm viability. Results are expressed as Tukey’s whisker boxes (median, 25th to 75th percentiles ± 1.5 interquartile range). Extreme values are represented individually according to the experimental group (filled circle CTRL—standard chow; filled square HFD—high-fat diet; filled triangle HFDt—transient high-fat diet). Epidydimal sperm was obtained from mice immediately after sacrifice, at 200 days post-weaning. Data was tested by one-way ANOVA with Tukey’s HSD for group comparison within the same generation. Two-way ANOVA with Šidak correction was used to compare each diet group in F1 Generation against its F2 counterparts. Graphs were obtained using the software GraphPad Prism 8 (San Diego, CA, USA). Significance was considered when p < 0.05. * vs. CTRL; # vs. HFD; § vs. F1 Generation. Multiple significance signs represent different significance levels: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. + Group mean.
