Figure 4
From: Cytochrome P450-epoxygenated fatty acids inhibit Müller glial inflammation
The effect of 11,12-EET and 19,20-EDP on IL-1β-induced inflammatory cytokine expression by Müller cells. Human Müller cells were treated with IL-1β (1.0 ng/ml) or IL-1β plus 11,12-EET (0.5 μM) or 19,20-EDP (0.5 μM) for 8 h. Total RNA was isolated and cytokine expression was assayed by qRT-PCR. (a) TNFα expression was significantly elevated while (b) IL1β, (c) IL6, and (d) IL8 expression was significantly decreased by the addition of both exogenous epoxygenated fatty acids. Results depicted are representative of three separate experiments. These data are normalized to induction levels illustrated in Fig. 5. Data are displayed as mean ± SD (n = 3).
