Figure 5
From: Cytochrome P450-epoxygenated fatty acids inhibit Müller glial inflammation
The effect of sEH inhibitor GSK2256294 on IL-1β-induced inflammatory mediator expression by Müller cells. Human Müller cells were treated with IL-1β (1.0 ng/ml) or IL-1β plus 0.1 nM, 1.0 nM, or 10 nM GSK2256294 (sEH inhibitor) for 8 h. Total RNA was isolated and cytokine expression was assayed by qRT-PCR. (a) TNFα, (b) IL1β, (c) IL6, and (d) IL8 expression was significantly decreased at each sEH inhibitor concentration tested. Bars represent mean ± SD (n = 6).
