Figure 3
Diazepam-treated Ts65Dn mice show genotype-dependent differences in neural synchrony. (a, b) Histograms of phase differences between cortical and hippocampal EEG recordings, which were computed by dividing the phase-spectrum (0–360°) into equal-sized 100 bins (3.6°/bin). (a) Control and Ts65Dn mice showed similar phase-lag distributions during baseline recordings. (b) Diazepam at 1-mg/kg dose narrowed the peak of phase-difference distribution in control mice as the number of phase-lag estimates close to zero increased, whereas phase lag distribution of diazepam-treated Ts65Dn mice exhibited an almost uniform distribution. (c) Diazepam-treated control mice showed an increase in the pairwise coherence in the beta-2 frequency range (19.40–23.20 Hz; grey asterisks) compared to their pre-injection baseline. (d) Diazepam-treated Ts65Dn mice showed no significant changes in coherent activity compared to their pre-injection baseline. Significant differences in pairwise coherence at 20.60–23.40 Hz and 43.20–44.60 Hz frequency ranges were detected between diazepam-treated control and Ts65Dn mice (black asterisks in c). Number of mice: Ct baseline (N = 6), Ct 1 mg/kg diazepam (N = 6), Ts baseline (N = 7), and Ts 1 mg/kg diazepam (N = 7).
