Figure 4
From: Dermal bacterial LPS-stimulation reduces susceptibility to intradermal Trypanosoma brucei infection
Enhanced ability of LPS-stimulated RAW264.7 cells to kill T. brucei. (A) Macrophage-like RAW264.7 cells were stimulated with 10 µg LPS (or PBS as a control), co-cultivated with T. brucei STIB 247 parasites, and the number of viable parasites determined 24 h later. (B,C) Macrophage-like RAW264.7 cells were stimulated with 10 µg LPS and/or 1 mM indolepyruvate, and then co-cultivated with T. brucei STIB 247 parasites. Twenty four hours later (B) nitrite levels in the culture supernatant, and (C) the number of viable parasites were determined. Data are shown as the mean ± SD for 3 wells/group from an individual experiment, and are representative of data from 3 independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; # below the limit of detection.
