Figure 1
From: Intracellular localization of the mycobacterial stressosome complex
Schematic representation of domains of RsbR and RsbS proteins and design of several deletions along with the protein–protein interaction results of the bacterial two-hybrid (BTH/BACTH) assay. (a) The RsbR protein (298 amino acids long) contains a C terminal "STAS" domain (amino acids 174–285) and five (numbered 1 to 5) major coiled-coil (CC) regions distributed along the polypeptide as revealed using the "coils" tool available on Expasy. The amino acid numbers for start and end of ‘CC’ regions are as follows: 1. 30–43; 2. 83–96; 3. 123–136; 4. 159–173; 5. 275–289. Several RsbR deletions (names are given on the right-hand side of every scheme) are also shown along with their BTH (BACTH) interaction results with RsbR, RsbS and RsbT proteins. DN and DC refer to deletion at the N- and C-terminals, respectively. (b) The RsbS protein (129 amino acids long) mostly consists of a "STAS" domain (first 112 amino acids). The deletion scheme shows progressive 43 amino acids deletion either from N terminus (RsbSDN1 and RsbSDN2) or from the C terminus (RsbSDC1 and RsbSDC2). Right panel shows a summary of the BTH (BACTH) results. Note that full length RsbS is required for its interaction with RsbR and RsbT. In both panels A and B, plus and minus signs refers to interaction or no interaction based on Fig. S4. For details see main text.
