Figure 2
From: Intracellular localization of the mycobacterial stressosome complex
Localization of the "stressosome" subunits RsbR and RsbS in MmarT detected by mCherry and GFP fusions. (a) Represents MmarT strains carrying empty pBS401-mCherry (red) and pST2K-GFP (green) plasmids (first column to the left), the pBS401-RsbR-mCherry (red) and pST2K-RsbS-GFP (green) at exponential (2 days) and stationary phase (10 days) in second and third columns to the right. The foci of RsbR and RsbS localization is shown by white arrows, scale bar = 1 μm. (b) Represents the various classification of localization for statistics based on the position and number of spots (localizations) in a cell, scale bar = 1 μm. (c) Shows the average percentage distribution (with errors) of the different types of localizations of RsbR and RsbS in exponential and stationary growth phases with respect to total number of cells considered. As shown, most of the cells showed dispersed fluorescence with RsbR-mCherry in both exponential and stationary phase (blue bars). A total of two biological replicates were considered and minimum of 300 cells were counted for each time point/condition. The y-axis represents the average percentage of different "cell types" of total number of counted cells.
