Figure 2
Identification of putative sex chromosome scaffolds. Sequencing data were subsampled to \(\sim\) 39 Gb and mapped to pseudo-haplotype1. Mean mapped read depth of the 10x Genomics reads produced in this study (SRX7520800; green shaded area), and the female (black line with open diamonds) and male (purple line) Illumina reads from Hazzouri et al.18 (SRX5416728 and SRX5416729) is shown in 100 kb windows across the 10 longest scaffolds of pseudo-haplotype1 assembly with terminal windows removed. Phase blocks are shown as gray rectangles. The ratio of male/female mean mapped read depth is given on the right side of each scaffold. Scaffolds with a male/female ratio of \(\sim\) 0.5 are indicated as putative sex chromosome sequences. The similar mapped read depth of our RPW sample and the female sample from Hazzouri et al., as well as the presence of phase blocks on putative sex chromosome scaffolds implies heterozygosity due to diploidy and indicates that such scaffolds are X-linked and that the individual sequenced in this study is female.
