Figure 2
RNA in situ hybridization of IFNG and TRDC transcripts. Follicular recto-anal junction (RAJ) isolated from a representative animal which received no adjuvant or vaccine strain (NoAdj-NoVac) (A) depicting mucosal epithelium (left panel) and submucosa-follicle (right panel). Follicular RAJ isolated from a representative animal which received non-adjuvanted-Δhha-E. coli vaccine (NoAdj-Vac) (B) depicting mucosal epithelium (left panel) and submucosa-follicle (right panel). Follicular RAJ isolated from a representative animal which received adjuvanted-Δhha-E. coli vaccine (Adj-Vac) (C) depicting mucosal epithelium (left panel) and submucosa-follicle (right panel). Graphical representation of γδTCR+ (right) and IFNγ+ (left) from mucosal epithelium (D), submucosa lumen (E) and submucosa-follicle (F). On inset panels indicated in (A–C), an approximate 5 × magnification section is represented whereas larger panels are approximately 20 × magnification of the inset. Black arrows indicate γδ T cell staining (specifically, the T-cell receptor, delta chain RNA) and blue arrows indicate IFNγ (interferon RNA) staining. Open circles indicate NoAdj-NoVac, grey circles represent NoAdj-Vac, and black circles represent Adj-Vac group. Each symbol represents an individual animal. N = 4 per group. Bars = ± SD. Bar data represents percentage of cells measured by RNA staining. One-way ANOVA with Tukey’s post-test was utilized for statistical analysis. ****p < 0.001, *p < 0.05.
