Figure 3
Increased abundance of IFNγ-positive cells in RAJ of cattle receiving adjuvanted vaccine. Characterization of cells within follicles of the recto-anal junction by immunofluorescent staining for either γδ TCR or IFNγ (A, B). Follicular region of RAJ was sectioned and labeled with florescent antibodies directed toward IFNγ (red) or γδTCR (green) and stained with nuclear stain DAPI (blue). Dashed lines indicate follicle boundary and hashed box indicate magnified section shown in (B) with representative section as indicated from an animal in non-adjuvanted-non-vaccinated (NoAdj-NoVac; NA-NV), non-adjuvanted-vaccine (NoAdj-Vac; NA-V), or adjuvanted-vaccine (Adj-Vac; A-V) groups. (C) Graphical representation of florescent data from indicated groups. Bars are ± SD. ****p < 0.0001, **p < 0.01, *p < 0.05 one-way ANOVA with Tukey’s post-test.
