Figure 5 | Scientific Reports

Figure 5

From: Mucosal IFNγ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle

Figure 5

Treatment of epithelial cells with IFNγ limits attachment of O157. HEp-2 cells (A) and bovine intestinal epithelial cells (BIEC) (B) were either untreated (Medium only, open circles) or pretreated with recombinant bovine IFNγ (IFNγ, black circles) and subsequent O157 attachment assessed as described in Materials and Methods. (A) Results presented are the average of two independent assays, and each assay included four wells. Bars are ± SD. **p < 0.01 determined by One-way ANOVA with Dunnet posttest, (B) The data is expressed relative to average attached O157 in media-only wells. Data is representative of two biological experiment (n = 2) and three technical replicates.

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