Figure 3

In vivo kinetics of luciferase expression from PEG-3-fluc plasmid formulated nanoparticles. Luciferase expression from PEG-3-fluc nanoparticles (chequered columns) was followed by bioluminescence imaging (at injection, 0 h; and at 24 h intervals up to 96 h) and compared to CMV-fluc (positive) (filled columns) and vehicle (negative) controls in mice inoculated with B16F10 tumor cells (A). At termination, bioluminescence in the resected lungs was measured ex vivo (B). Both in vivo and ex vivo imaging of the treated mice showed detectable expression of luciferase in both the PEG-fluc and CMV treated mice by 24 h, which continued for at least 96 h.