Figure 1
(a) Experimental setup for combined two-photon imaging and infrared stimulation. (b) Two-photon image of the area recorded showing cells targeted by infrared radiation. The arrow indicates the cell whose traces are shown in (c) Scale bar: 100 µm. (c) Representative example of infrared-induced intracellular calcium change in a single cell over 5 consecutive trials (0.58 J cm−2). Bottom panel, average of the 5 recordings with 5 consecutive identical stimuli. In black: the average of the repetition of the five recordings reproduced with the same infrared exposure parameters; in grey: standard deviation. The arrowhead marks the beginning of the infrared exposure (figure created from Powerpoint 2016, MESc software and Matlab R2018b).
