Figure 2

(a,b) Representative two-photon field of view of a single mouse visual cortex showing responsive identified cells (white contours) and non-responsive cells (yellow contours), with adjusted lookup table in (b). for better visualization. The arrows denote the two cells taken as example in (c) and (d) Scale bar: 100 µm. (c,d) Examples of 5 recordings average GCaMP6f fluorescence time course in a single cell not responding and responding, respectively, to the infrared exposure (marked, respectively, by white and yellow arrows on a and b). Black line: average of the repetition of the five recordings reproduced with the same infrared exposure parameters (0.70 J cm⁻²); grey: standard deviation. The black arrow marks the beginning of the infrared exposure. Red lines represent the threshold used in all figures and data analysis to identify responding and not responding cells (see “Methods” for thresholds determination) (figure created from MESc software and Matlab R2018b).