Figure 3
From: Cage and maternal effects on the bacterial communities of the murine gut
Random forest model identifies strong associations between the microbiota, niche, age and cage. The predictive accuracy of the random forest model at taking a sample and discriminating between the different treatment groups is shown (a). The five most important nodes associated with microbial niche (b), host age (c), social group (cage) (d) and mother (e), are named based on the finest scale taxon containing the closest BLAST hits of all sequences in the clade. Taxa are prefixed with their taxonomic level: order (o_), family (f_) and genus (g_). Bars represent means and standard errors. Asterisks represent significance determined using Two Way ANOVA-Sidak’s post hoc: P < 0.00001 (****). Similar plots for further validation control analyses are shown in Supplementary Figure S3 and S4. n = 6 technical replicates. Figure produced in GraphPad Prism 8.
