Figure 1
From: Structural insight into Pichia pastoris fatty acid synthase
Determination of P. pastoris FAS structure by cryoEM. (a) Raw micrograph showing VLPs with contaminating FAS particles. FAS particles are indicated by white arrows. Scale bar shows 100 nm. (b) The most populated classes from 2D classification of P. pastoris FAS using a box size of 460 pixels (after excluding ‘junk’ classes of FAS over thick carbon support) (upper) compared with 2D class averages of S. cerevisiae FAS showing partial denaturation (indicated by red arrows) from D’Imprima et al. (2019)19 (licensed under CC BY 4.0) (lower). (c) Orientation distribution map of particles contributing to the final reconstruction of the FAS asymmetric unit. The map shows the probability that any particle was assigned the orientation indicated, according to the scale shown. (d) CryoEM density map of P. pastoris FAS, coloured radially.
