Figure 3

Unique structural features of P. pastoris FAS. (a) Overlay of P. pastoris FAS (orange) and S. cerevisiae FAS (PDB 6TA1, blue) from Joppe et al. (2020)²⁰, with differences highlighted by stronger colours. Enlarged images are indicated on the map with boxes. (b) Wire representation of atomic model for P. pastoris FAS (orange) overlaid with a range of models of S. cerevisiae FAS (PDB 6TA1²⁰, 6QL9²¹, 6QL6²¹, 2UV8³⁰; blue), with regions of high deviation (RMSD ≥ 15 Å) coloured red for S. cerevisiae FAS models. Segments of the backbone that were obviously misaligned (e.g., due to missing residues) were removed for clarity. (c) Loop region comprising residues 1753–1768, highlighting the different positioning compared to the equivalent region from S. cerevisiae FAS. The P. pastoris density map is overlaid at high contour level (left) and low contour level (right). (d–f) Other regions from different parts of the complex are highlighted and compared with equivalent regions of S. cerevisiae FAS, including the loop comprising residues 914–919 (d), the beta strand comprising residues 1553–1558 (e) and the additional resolved linking region comprising residues 1113–1135 (f).