Figure 3

Plots of the relative FRET efficiency (E_rel) as a function of pressure for the dually labelled 22AG in the absence (black squares) and in the presence (red circles) of ThT (ThT:22AG ratio = 10:1) in (A) 30 mM Tris, pH 7.4, (B) in the presence of 60 mM NaCl, (C) in the presence of 60 mM KCl, and (D) in the presence of 25 wt% Ficoll. All the experiments were carried out in triplicate at 25 °C. Where not shown, the error bars are smaller than the symbol size.