Figure 4

Binding isotherms for the 22AG/ThT system in (A) 30 mM Tris, pH 7.4, (B) in the presence of 60 mM NaCl, (C) in the presence of 60 mM KCl, and (D) in the presence of 25 wt% Ficoll at the pressures of: 1 bar (black squares), 500 bar (red circles), 1000 bar (blue triangles), 1500 bar (green reversed triangles), and 2000 bar (violet diamonds). All the experiments were performed in triplicate at the temperature of 25 °C. Where not shown, the error bars are smaller than the symbol size.