Figure 5

Pressure dependence at T = 25 °C of the natural logarithm of the binding constant, lnK_b, in 30 mM Tris pH 7.4 (black squares), in the presence of 60 mM NaCl (red circles), in the presence of 60 mM KCl (blue triangles), and in the presence of 25 wt% Ficoll (green reversed triangles). From the fit of lnK_b vs. p, the reaction volume (ΔV_r) can be determined. Where not shown, the error bars are smaller than the symbol size.