Table 2 Relative FRET efficiency (Erel) for the various conformations of 22AG as inferred from the comparison of the FRET and CD data at p = 1 bar and T = 25 °C.

From: Unraveling the binding characteristics of small ligands to telomeric DNA by pressure modulation

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Conformation

Erel

Unfolded

 ~ 0.33

Antiparallel

 ~ 0.81

Parallel

 ~ 0.47

Hybrid-1

 ~ 0.88

  1. The scheme at the top depicts the quadruplex structures adopted by the 22AG sequence: (A) the antiparallel, (B) the parallel, and (C) the hybrid-1 conformation. In the labeled sequences, the donor (FAM) is located at position 5′, and the acceptor (TAMRA) in position 3ʹ.
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