Figure 4
Changes in mitochondrial oxidative phosphorylation in the fetal cortex after maternal hyperoxia. (A) Representative immunoblots showing all 5 electron transport chain complexes in the cerebral cortical homogenates of both male and female offspring after maternal exposure to either room air or hyperoxia, with rat heart mitochondria as positive control. Exposure time had to be increased from 1 to 3 min to visualize Complex I. (B) Scatter plots highlighting the lack of significant changes in mitochondrial OXPHOS proteins in the fetal brain after exposure to 6 h of maternal hyperoxia with 100% oxygen. Data are presented as mean ± S.E.M and analyzed with 2-way ANOVA with Sidak’s multiple comparisons test to assess for sex differences in the treated group (n = 3 each for all experiments).
