Figure 1
From: Use of single guided Cas9 nickase to facilitate precise and efficient genome editing in human iPSCs
DN, but not SN, promotes indel formation in human iPSCs. (A) Schematic representation of two gRNA sequences (on two opposite strands) upstream of the CAG repeat in exon 7 of human PPP2R2B gene. Primers F1 and R1 were used for PCR amplification. (B) Indel frequencies calculated by TIDE using sequence traces. BCL-XL overexpression increased indels induced by DN but did not increase indels induced by SN. Mean and SEM. N = 3 independent experiments. One-way ANOVA F(3,8) = 150.0; p < 0.001; Tukey’s multiple comparison test ***p < 0.001.
