Figure 5
From: Use of single guided Cas9 nickase to facilitate precise and efficient genome editing in human iPSCs
Targeting efficiency of the mutant allele in human SCA12 iPSC lines by SN. (A–C) Gel images of 5′ and 3′ junctional PCRs in puromycin-resistant clones from three SCA12 (122i, 380i, and 515i) iPSC lines processed with SN and BCL-XL. (D). Summary of targeting efficiency in three SCA12 iPSC lines.
