Figure 2

Localisation of H3R2me2s in pronuclei of zygotes. (a) Immunostaining for localisation of H3R2me2s in MII oocytes and PN1–5 stage zygotes. The representative images of zygotes stained with DAPI (blue) and anti-H3R2me2s antibody (red). (b) An image example showing the male pronucleus of a zygote at PN5. Male pronuclei corresponding to dashed squares are shown magnified below. Shown are representative images of zygotes stained with DAPI (blue) and anti-H3R2me2s antibody (red). Key: ♂, male pronuclei; ♀, female pronuclei; PB, polar body; arrowhead, sperm. Scale bar = 50 µm.