Figure 3 | Scientific Reports

Figure 3

From: Symmetrically dimethylated histone H3R2 promotes global transcription during minor zygotic genome activation in mouse pronuclei

Figure 3

Reduction of transcriptional activities by expression of H3.3R2A mutant in zygotes. (a), (c) Immunofluorescent images of BrUTP (red), Pol II Ser2P (red), and DAPI (blue) in H3.3-EGFP-expressed, H3.3R2A-EGFP-expressed, and untreated zygotes. EGFP (green) shows successful injection. Key: ♂, male pronuclei; ♀, female pronuclei. Scale bar = 20 µm. (b), (d) Quantification of the BrUTP and Pol II Ser2P signal intensities in pronuclei of H3.3-EGFP-expressed, H3.3R2A-EGFP-expressed, and untreated zygotes. Red bars indicate the mean values. The mean value of male pronuclei in untreated zygotes was set as 1. One-way ANOVA and the Tukey–Kramer test were performed to evaluate statistical significance. *Significantly different from the control (*p < 0.05, **p < 0.01); n.s.: Not significantly different from the control. The number of zygotes in (b) was 10 for the H3.3-EGFP expressed group, 24 for the H3.3R2A-EGFP expressed group, and 21 for the untreated group. The number of zygotes in (d) was 15 for the H3.3-EGFP expressed group, 13 for the H3.3R2A-EGFP expressed group, and 10 for the untreated group. The pronucleus used for quantification are presented in Fig. S7a, b.

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