Figure 4 | Scientific Reports

Figure 4

From: Symmetrically dimethylated histone H3R2 promotes global transcription during minor zygotic genome activation in mouse pronuclei

Figure 4

Downregulation of MuERV-L via expression of H3.3R2A mutant in early 2-cell at 24 hpi. (a) Representative images of RT-PCR of MuERV-L and Armoured RNA control (External control). (b) Quantification of the PCR bands. The mean value of male pronuclei in untreated zygotes was set as 1. Mean ± S.E. from three independent experiments. One-way ANOVA and the Tukey–Kramer test were performed to evaluate statistical significance. *Significantly different from the control (***p < 0.001); n.s.: Not significantly different from the control. The full-length gels used for quantification are presented in Fig. S8.

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