Figure 5 | Scientific Reports

Figure 5

From: Symmetrically dimethylated histone H3R2 promotes global transcription during minor zygotic genome activation in mouse pronuclei

Figure 5

Reduction of transcriptional activities by DS-437-treatment in zygotes. (a) Schematic diagram of the experimental procedures. (b), (d), (f) Immunofluorescent images of H3R2me2s (red), BrUTP (green), Pol II Ser2P (green), and DAPI (blue) in DMSO- and DS-437-treated zygotes. DMSO treatment was used as a control. (c), (e), (g) Quantification of the H3R2me2s, BrUTP, and Pol II Ser2P signal intensities in pronuclei of DMSO- and DS-437-treated zygotes. Red bars indicate the median values. The median value of male pronuclei in untreated zygotes was set as 1. Mann–Whitney U test were performed to evaluate statistical significance. The number of zygotes in (c) was 14 for the DMSO-treated group and 28 for the DS-437-treated group. The number of zygotes in (e) was 9 for the DMSO-treated group and 12 for the DS-437-treated group. The number of zygotes in (g) was 10 for the DMSO-treated group and 16 for the DS-437-treated group. The pronucleus used for quantification are presented in Fig. S9.

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