Figure 6 | Scientific Reports

Figure 6

From: Symmetrically dimethylated histone H3R2 promotes global transcription during minor zygotic genome activation in mouse pronuclei

Figure 6

Knockdown of Prmt5 and Prmt7 mRNA revealed reduction in global transcription activities during minor ZGA. (a) Schematic diagram of the experimental procedures. (b) Knockdown of Prmt5 and Prmt7 expression by an antisense RNA injection was confirmed by quantitative RT-PCR analysis. The relative ratios were obtained by dividing the expression level of the Prmt5 and Prmt7 with the expression level of the Gapdh. Student’s t-test were performed for gel images. *Significantly different from the control (***p < 0.001). Bars represent the standard error of the mean. Full-length gels are presented in Fig. S10a. (c) Immunofluorescent images of H3R2me2s (red), Pol II Ser2P (green), and DAPI (blue) in Prmt5 and Prmt7 knockdown zygotes. siControl RNA was used as a control. (d), (e) Quantification of the H3R2me2s and Pol II Ser2P signal intensities in pronuclei of Prmt5 and Prmt7 knockdown zygotes. Dunnet test were performed to evaluate statistical significance. The number of zygotes in (d), (e) was 13 for the siControl group, 13 for the siPrmt5 group, 10 for the siPrmt7 group, and 13 for the siPrmt5&7 group. The pronucleus used for quantification are presented in Fig. S10b.

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