Figure 3
Effect of JAK3 inhibitors on IL-4 induced STAT6 phosphorylation. BMDMs were isolated from wild type and JAK3 Cys905Ser knockin mice. Cells were incubated with the indicated concentrations of inhibitor before stimulation for 30 min with 10 ng/ml IL-4. Cells were lysed and the levels of STAT6 phosphorylated at Tyr641 were assessed by immunoblotting. Levels of ERK1/2 were determined as a loading control. Data is shown for the JAK3 inhibitors TL6-144, TL-852, PF-06651600 and FM-381. Representative blots are shown in (A) and quantification of two independent experiments is shown in (B) and calculated IC50 values in (C).
