Figure 5 | Scientific Reports

Figure 5

From: Reduced competence to arboviruses following the sustainable invasion of Wolbachia into native Aedes aegypti from Southeastern Brazil

Figure 5

Wolbachia inhibits ZIKV and DENV tissue replication following oral-infection of field samples. Following the long-term establishment of Wolbachia in Jurujuba, field samples were brough to the laboratory and orally challenged with ZIKV and DENV, either fresh or frozen. Wolbachia-mediated inhibition of ZIKV and DENV replication was evaluated by comparing samples from Jurujuba (green) and Urca (grey), a Wolbachia-free non-targeted area in the suburbs of Rio de Janeiro. Violin plots represent absolute quantifications of (A) DENV and (B) ZIKV titers in head/thorax extracts, at 14 dpi. Median and quartiles are depicted in solid and dashed red lines, respectively. The proportion of infected individuals are shown as fractions underneath. Wolbachia– Jurujuba individuals were flagged by empty dots. Non-parametric Mann–Whitney U test highlighted differences between Urca and Jurujuba. Asterisks denote significant effects, with α equal to 0.05, and levels varying according to probability ranges: *P < 0.05, **P < 0.01, ***P < 0.001. Graph and statistics were made with GraphPad Prism 8 (https://www.graphpad.com).

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