Figure 5

AFM-based ultrastructural analysis of the epidermal cell wall of Senna alexandrina (Fabaceae) leaves. AFM-data acquisition on cell wall ultra-sections allowed nanoscopic resolution down to the microfibril level. (a–c) Low, medium and high magnification amplitude data of Senna alexandrina cell wall morphology. Note the clear depiction of the layered cell wall composition and the resolution of single macro- as well as microfibrils (white triangle). (d) High resolution height data of single microfibrils (3D representation). (e) Section analysis of a single microfibril showing lateral dimensions of just below 10 nm. Note the corresponding z-axis resolution below 0.5 nm. Scale bars (a) 4 µm, (b) 1 µm, (c) 320 nm, (d) 100 nm. Cut cuticle, CW cell wall, white triangle isolated microfibril.