Figure 4
From: Differential mast cell numbers and characteristics in human tuberculosis pulmonary lesions
Tryptase and chymase positive mast cells are numerous in fibrotic lesions of tuberculous lungs. (A) Representative low power micrograph of fibrotic tuberculous nodule stained with HE and immunofluorescence micrographs from the same lesion shows MCT (green), MCC (red) and MCTC (merge). (B) MCs subtypes counted in ten different high-power fields (microarrays) (40 ×) from 44 autopsy cases confirm that MCTC were the most abundant phenotype at fibrotic areas. (C) High power micrograph at fibrotic areas shows MCs that express tryptase and chymase cytoplasmic granules which are partially degranulated. Shapiro–Wilk test was done to determine normality. Representative section of a fibrotic area was incubated with anti-TGF-β (Alexa 647 label) and anti-tryptase antibodies (Alexa 488 label). (D) High power micrograph shows TGF-β colocalization with MCT. Statistical comparison was performed using Kruskal–Wallis test and Dunn´s multiple comparison post-test (adjusted *p ≤ 0.01).
