Figure 5 | Scientific Reports

Figure 5

From: Iron supplementation regulates the progression of high fat diet induced obesity and hepatic steatosis via mitochondrial signaling pathways

Figure 5

Iron supplementation reduces mitochondrial morphological abnormalities and increases mitochondrial-associated gene expression. (a–i) Male C57BL/6J mice of 6 weeks of age were fed with each diet for 15 weeks as specified in Fig. 1. (a) Representative image of transmission electron microscopy analysis in skeletal muscle. Yellow arrows point to the mitochondria. Scale bar is 1 μm. (b, c) Copy number of mitochondrial DNA (mtDNA) in (b) liver and (c) skeletal muscle (n = 5–6). (d, e) ATP levels normalized by tissue weight in (d) liver and (e) skeletal muscle (n = 5–6). (f, g) ATP levels normalized by mtDNA in (f) liver and (g) skeletal muscle (n = 5–6). (h, i) Analysis of mRNA expression related to fatty acid beta oxidation (β-ox), mitochondrial (Mt), mitochondrial respiratory chain complex I (I), II (II), III (III), IV (IV), and V (V) in (h) liver; and (i) skeletal muscle (n = 5–6). Data are expresses as mean ± SEM values. Statistical analysis was performed using a one-way ANOVA followed by Holm–Sidak’s post hoc test. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant. (Significant differences vs. HF).

Back to article page