Figure 4
From: Maternal control of visceral asymmetry evolution in Astyanax cavefish
The expression of lefty genes in surface fish and cavefish embryos during segmentation. (A–D) In situ hybridization showing lft1 expression along the dorsal midline in 10–13 somite surface fish (A, B) and cavefish (C, D) embryos. (A, C) Dorsal views with anterior on the top. (B, D) Lateral views with anterior on the left. (F–H) In situ hybridization showing lft2 expression in the LPM (arrows) in 18–25 somite surface fish (F) and cavefish embryos (G, H). Dorsal views. The numbers in the frames indicate embryos with the indicated expression pattern compared to the total number of in situ hybridized embryos. Scale bar in (B): 100 µm; magnification is the same (A–D) and (F–H). (E) Bar graph showing relative fold changes in lft1, lft2, and spaw mRNA levels in cavefish compared to surface fish determined by qPCR at the 18–25 somite stage. Black bars: surface fish. Yellow bars: cavefish. Error bars: SEM. Number of replicates shown at the bottom of the columns. Asterisk: p < .01.
