Figure 1 | Scientific Reports

Figure 1

From: Mouse IgG3 binding to macrophage-like cells is prevented by deglycosylation of the antibody or by Accutase treatment of the cells

Figure 1

Deglycosylation of mIgG3 prevents its binding to J774A.1 and P388D1 cells. (a) Erythrocyte rosetting around J774A.1 or P388D1 cells coated with the M18 mIgG3 antibody specific to antigen B of the human ABO blood group system. The cells were coated with native or deglycosylated M18 (degM18). The small cells are group B human erythrocytes, whereas the large bright cells are J774A.1 or P388D1 cells. The arrows point to exemplary rosettes. Representative microscope images of three independent experiments are shown. The pie charts display the percentage of rosetting and non-rosetting cells observed in three different fields in the same representative experiment. (b,c) Binding of M18-488 to J774A.1 or P388D1 cells analyzed using flow cytometry. The cells were stained with fluorescently labeled native or degM18. The dot plots show representative results of three independent experiments. (c) Average values ± SD of the three independent experiments described in (b).

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