Figure 4

adopted from Esch et al., 2013; Jerabek et al., 2014). (B) and (C) EMSA experiments examining the binding of purified recombinant His-OCT4 (WT and K222 variants) proteins singly or in combination with recombinant SOX2 proteins, to biotinylated SORE, PORE and MORE probes. (D) HeLa cells were co-transfected with different OCT4-binding motif-luciferase reporter constructs and the WT OCT4 construct with the empty vector as a control, relative mRNA levels of the expressed OCT4 and luciferase were determined by qRT-PCR 48 h after transfection. (E) HeLa cells were co-transfected with different OCT4-binding motif-luciferase reporter constructs and the OCT4 constructs (WT and K222 variants) with the empty vector as a control, the relative luciferase mRNA levels were determined by qRT-PCR 48 h after transfection. Results shown in (D) and (E) were presented as means ± S.D. of triplicate measurements from single experiment representative of 3 independent experiments. Two-tailed unpaired Student’s t tests were used for statistical analyses. *P < 0.05 and **P < 0.01.