Figure 5

OCT4-K222 methylation represses the transcription of PORE-motif-containing target genes in vivo and the differentiation capacity of PSCs. (A) The morphology of H9 cells 72 h after transfection with shOCT4-FLAG-OCT4 (WT and variants) constructs. (B) A brief summary of PORE genes’ main functions. (C) H9 cells were transfected with shOCT4-FLAG-OCT4 (WT and variants) constructs with the empty vector as a control. 72 h later, cells were harvested and the relative mRNA levels of the PORE genes listed in (B) were determined by qRT-PCR. (D,E) The relative mRNA levels of the stemness genes and lineage-specific marker genes in H9 (D) and H1 (E) cells subjected to the same transfection procedures as in (C) were determined by qRT-PCR. Results shown in (C), (D) and (E) were presented as means ± S.D. of triplicate measurements from single experiment. Two-tailed unpaired Student’s t tests were used for statistical analyses. *P < 0.05 and **P < 0.01.