Figure 1
BP1026B_II1198 is differentially regulated during cell infection and critical for intracellular survival. (a) Single cell ‘transitome’ analysis7 shows that BP1026B_II1198 is up-regulated in the vacuole and protrusion while down-regulated in the cytoplasm during infection of RAW264.7 cells. Boxes represent three biological replicates and an average (Ave)7. (b) A mutant in BP1026B_II1198 shows a reduced ability to form wildtype level MNGCs. When the mutant is complemented it recovers beyond wildtype level of MNGC size. Individual MNGC diameters are shown as dots. (c) The BP1026B_II1198 mutant shows a significant reduction in intracellular replication at 8 and 24 h after infection compared to wildtype Bp 1026b. Complementation of the BP1026B_II1198 mutant recovers the intracellular replication to wildtype levels. (d) The BP1026B_II1198 mutant and complement grow to similar levels of wildtype Bp 1026b in vitro. Data in bar graphs and growth curve represent means ± s.e.m and analyzed via unpaired t-test. P values presented are as follows: ** p < 0.01, ****p < 0.0001.
