Figure 4
Genes highly regulated by BP1026B_II1198 show involvement during Bp intracellular survival. (a) Normalized transcripts of genes showing a log2 FC ≤ -2 or ≥ 2 in the presence of BP1026B_II1198 compared to an empty vector control. (b) Predicted functions of genes controlled by BP1026B_II1198. Percent of wildtype replication (%WT) in RAW264.7 cells is shown 24 hpi. Several transposon mutants were not available in the sequence-defined transposon mutant library of Bp 1026b (NA). (c) Transposon mutants present in the sequence defined transposon mutant library of Bp 1026b were analyzed for the ability to replicate in RAW264.7 murine macrophages for 24 h. Intracellular replication represented as a percent of wildtype 24 hpi and the average of triplicates represented in (b). (d) Schematic diagram showing genes involved in Bp intracellular survival (replicating < 80% wildtype) by indirect control of BP1026B_II1198. Data in bar graphs represent means ± s.e.m and analyzed via unpaired t-test. P values presented are as follows: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
