Figure 6
BP1026B_II1198 has direct transcriptional control of genes involved in pathogenesis of RAW264.7 murine macrophages. (a) Schematic representation of the direct regulation network of BP1026B_II1198. BP1026B_II1198 binds to an intergenic region (peak 1) between BP1026B_II0600 and BP1026B_II0601 and an intergenic region (peak 2) between BP1026B_II1006 and BP1026B_II1007. (b) Normalized transcripts of genes surrounding peak 1 identifies that BP1026B_II1198 positively regulates BP1026B_II0600. (c) Normalized transcripts of genes around peak 2 identify that BP1026B_II1198 negatively regulates BP1026B_II1006. (d) Infection of RAW264.7 cells by transposon mutants of BP1026B_II0600 and BP1026B_II1006 compared to wildtype Bp 1026b showing various levels of intracellular replication defect. (e) Annotated function of BP1026B_II0600 and BP1026B_II1006 and corresponding intracellular replication when compared to wildtype Bp 1026b (%WT). (f) Model for the direct regulation network of BP1026B_II1198. Data in bar graphs represent means ± s.e.m and analyzed via unpaired t-test. P values presented are as follows: *p < 0.05.
