Figure 3
Sites and frequency of tumor distribution in the IC model. (a) Ex vivo bioluminescent images (BLI) of tissues to pinpoint the different organ sites of tumor cell colonization. Red boxes, liver; red asterisks, lung. (b) Quantified distribution of bioluminescent pNEN cells per tissue. All BON1.luc-injected mice exhibited lung tumors while all Qgp1.luc-injected mice exhibited liver tumors. (c) H&E images of Qgp1.luc and BON1.luc tumors in the liver, lung, adrenal and ovary tissues, as indicated in the inset of each image. Tumor cells are stained purple with discrete lesions (upper panels), scattered individual cells throughout the tissue (middle panels), or large lesions overtaking the normal tissue (bottom panels) shown. Blue arrow (top right panel) identifies a single, small BON1.luc liver tumor. (d) IHC staining (brown) for the NEN marker, synaptophysin, on the same tissues shown in (c).
