Figure 4

taken from 19 to 21 week old female miR-324-null and WT mice (N = 16 slices obtained from 6 mice per genotype). (a and b) Examples of traces following bath application of 150 nM KA that show large numbers of spikes (peaks more than 5SDs from the baseline) and IIDs, respectively (both measured in miR-324-null slices). The red dashed lines indicate 5 standard deviations above and below the baseline. (c and d) Electrophysiological traces of miR-324-null mice contained a significantly higher frequency of spikes and IIDs than WT control traces. The majority of slices obtained from WT mice showed no IIDs and therefore the frequency for these slices is zero. p Values were calculated using 2-way mixed ANOVAs of ranks, as the initial data was not normally distributed. *, ** and *** indicate p values ≤ 0.05, 0.01 and 0.001 respectively for post-hoc testing using Tukey’s HSD tests.