Figure 5
From: Increased hippocampal excitability in miR-324-null mice
Initial analysis of RNA-seq of hippocampus and neocortex in 13.5 month old male miR-324-null and WT mice. (a and b) PCA analysis does not show as strong a segregation of samples by genotype as it does by tissue, although the results of hippocampus and neocortex RNA-seq experiments are well correlated using RRHO (sample permutation p value < 0.001). (c) Only 40 of the genes found to be DE in miR-324-null samples relative to WT controls were found to be DE in both tissues. (d) Volcano plots for hippocampus and neocortex tissue showing the fold-change, adjusted p value of each gene in addition to whether it is a predicted target of either arm of miR-324 according to the TargetScan algorithm4,33. Genes significantly increased in expression in miR-324-null samples are shown in blue, genes significantly decreased in expression are shown in green and genes showing no significant changes (p value > 0.05) are coloured grey. Genes found to be significantly upregulated in miR-324-null hippocampus and neocortex, in addition to being a miR-324 target, are labelled. (e) Of the genes that were predicted targets of miR-324 and significantly upregulated in both the hippocampus and neocortex RNA-seq, only Cd300lf and Suox were significantly DE in all cohorts by qRT-PCR analysis. Sp6 was also highly upregulated, but it was detected in none of the WT samples in any cohort and so statistical significance could not be calculated. *, ** and *** indicate p values ≤ 0.05, 0.01 and 0.001 respectively, using two-sided Student’s t-tests to assess statistical significance.
