Figure 6

Relation of RNA-seq results to epilepsy. (a) Epilepsy syndrome was significantly enriched in the hippocampus RNA-seq, but not in the neocortex experiment (p values = 0.03 and 0.74 respectively), using terms and gene associations from the Disease Ontology. The significance of each Disease Ontology term was established using a Kolmogorov–Smirnov test. The dotted line on each plot shows where the cut-off for significance at a 5% level is found. (b) qRT-PCR quantification of the expression of epilepsy-associated genes was undertaken in the hippocampus tissue of the 13.5 month old male RNA-seq cohort and in 7 month old male and female hippocampal validation cohorts. * and ** indicate p values ≤ 0.05 and 0.01 respectively, using two-sided Student’s t-tests to assess statistical significance. Only Pnpo is significantly DE in all three cohorts.