Figure 1 | Scientific Reports

Figure 1

From: Therapeutically actionable signaling node to rescue AURKA driven loss of primary cilia in VHL-deficient cells

Figure 1

NVP-BEZ235 regulates ciliogenesis in VHL-deficient cells. (A) Workflow used for the image-based screening assay. (B) Graphical representation showing the Z-score of compounds used in the screen. The compounds annotated with names were identified as positive hits. (C) Chemical structure of NVP-BEZ235. (D) Representative images (60X magnification) of hTERT RPE-1 cells transfected with siC (scrambled control) or siVHL showing acetylated α-tubulin (cilia marker, green) and pericentrin (basal body marker, red). Nuclei are counterstained with DAPI in blue. Insets show a magnified image of a cilium for each of the treatment conditions. Scale bar, 10 μM. (E) Graphical representation of the percentage of ciliated cells treated with Vehicle or NVP-BEZ235 (Bez) at the indicated doses in VHL-proficient (siC, black line) and VHL-deficient (gray line) cells. *Statistical significance (Students t-test) compared to vehicle (DMSO) treated control (p < 0.01).

Back to article page