Figure 4 | Scientific Reports

Figure 4

From: Therapeutically actionable signaling node to rescue AURKA driven loss of primary cilia in VHL-deficient cells

Figure 4

Co-deletion of AKT and VHL rescues ciliogenesis. (A) Representative images of hTERT RPE-1 cells (60X magnification) transfected with siC (scrambled control), siVHL or co-transfected with siVHL and siAKT showing acetylated α-tubulin (cilia marker, green) and pericentrin (basal body marker, red). Nuclei are counterstained with DAPI in blue. Insets show a magnified image of a cilium for each of the treatment conditions. Scale bar, 10 μM. (B) Graphical representation of the percentage of ciliated cells for each of the treatments as indicated in VHL-proficient (siC, black line) and VHL-deficient (gray line) cells. **Statistical significance (Students t-test) compared to vehicle (DMSO) treated controls with indicated p-values. (C) Whole cell extracts from hTERT RPE-1 cells transfected with siC, siVHL or co-transfected with siVHL and siAKT, immunoblotted using antibodies shown.

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