Figure 3 | Scientific Reports

Figure 3

From: Intranasal immunization with inactivated chlamydial elementary bodies formulated in VCG-chitosan nanoparticles induces robust immunity against intranasal Chlamydia psittaci challenge

Figure 3

MOMP-specific antibody titers elicited after immunization. Groups of chickens were immunized IN or IM twice, 2 weeks apart as described above. Serum samples were obtained from 8 chickens/group on days 7 and 14 after the primary immunization, and again on days 7 and 14 after the booster dose. A MOMP-based antibody ELISA with twofold serum dilutions were used to assess the titer of antibodies elicited in serum after IN (A) and IM (B) immunization. Antibody titers were determined as the highest serum dilution that gave an optical density (OD450) above the cut-off value (mean negative OD values plus 2-SD). Results (one of two independent experiments with similar results) are presented as the reciprocal of the highest serum dilution with an absorbance above the cut-off value. Significant differences between experimental groups were evaluated by One-way ANOVA with Tukey’s post multiple comparison test at (p* < 0.01).

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